Powerful Performance: Combining the specificity of ELISA with the ultra-sensitivity of digital PCR, this platform achieves detection sensitivities on par with or exceeding Olink and Somalogic, with certain targets reaching SiMoA-level performance.
Flexibility: Suitable for both biomarker discovery and single-target validation. A wide selection of assay kits is available for human and animal models, meeting the needs of diverse research applications.
Ease of Use: Utilizing magnetic bead-based technology, the workflow is straightforward and requires no dedicated instrumentation—only a digital PCR system, which is readily accessible in most labs.
Rapid Turnaround: With a pre-run incubation of only 2.5 hours, single-analyte assays can be completed in as little as 4 hours.
Precise, Sensitive, Convenient
The Groundbreaking dIPCR ELISA Assay
The innovative Quantitative Immuno-PCR ELISA (dIPCR ELISA) platform, powered by Turtle Technology’s digital PCR systems, represents a significant advancement in protein quantification. It seamlessly merges the specificity of traditional ELISA with the exceptional sensitivity of digital PCR. Using a magnetic bead-based protocol, the system allows for accurate quantification of proteins across various sample types—including serum, plasma, and cell culture supernatants—with sensitivity reaching femtogram-per-milliliter levels. The platform supports a broad panel of human and animal models, offering robust and reliable solutions for both basic and preclinical research needs.
Advantages of the dIPCR Kit over Standard ELISA:
Ultra-High Sensitivity: Over 1,000x more sensitive than traditional ELISA, enabling precise detection of trace proteins.
Minimal Sample Volume: Requires less than 10 μL of sample, preserving precious materials.
High Specificity: Employs a sandwich-based design for precise target recognition and reliable quantification.
Simple Workflow: Only two processing steps.
Efficient Process: Full results in under 4 hours.
Simple 4-Hour Workflow Overview:
1. Immune Complex Formation and Purification:
In a 96-well plate, samples are incubated with DAB-ONA and CAB-biotin-SA magnetic beads. When the target protein is present, DAB-ONA binds to it and attaches to CAB-biotin-SA beads via biotin-streptavidin interaction. Unbound material is removed using magnetic washing.
2. PCR Amplification:
The immune complex is released from the beads and used as the dPCR template. PCR primers and master mix are added, and quantification is performed using a digital PCR system. Results are compared against a standard curve generated from known protein concentrations.
Product Specification:
Sensitivity vs. Olink or ELISA:
Product Catalog:
